Protein, Antibody and Small Molecule Arrays

Protein arrays are a powerful means to assess protein-protein interactions, monitor protein expression levels, or quantitate the amount of target analytes in biological samples.

At MI, we have significant experience in recombinant expression, protein folding and protein conformational integrity, which makes our company uniquely poised to advance your proteomics project. To achieve complex protein-protein interactions that mimic molecular associations found in vivo, several physical and biological factors must be taken into account on the array. Our expertise in immobilizing proteins and other small molecules onto a variety of array surface types provides our clients the opportunity to achieve results that cannot be obtained elsewhere.

For the detection of proteins, antibodies, and other analytes we offer specialized services such as reduced temperature-printing conditions, proprietary solubilization buffers, ranging deposition volumes, functional QC and product validation. Our systems readily accommodate both high- and low-density analysis formats, as well as high-throughput 96-well microplate formats commonly used in clinical diagnostics and drug discovery.

QC is a major consideration for any project. MI prides itself on its QC systems and will work with you to establish the most appropriate product validation strategy for your protein assays. For quality assurance purposes, MI can often incorporate an off-channel fluorophore, which will not interfere with subsequent analysis. This fluorophore can be used to monitor volumetric transfer and spotting morphology. MI can also perform QC on protein arrays using functional assays to validate array quality.

Whether your research requires a small number of proteins on thousands of arrays or thousands of proteins on a limited number of arrays, our flexible printing platforms allow every size project to be run efficiently and economically.

Pilot Projects

MI has extensive experience and expertise developing meaningful and specifically targeted pilot projects focusing on all aspects of array design and fabrication. MI scientists will work with you to identify factors that might impact the successful development of the desired array. Together, we will design well-reasoned pilot projects to identify the best substrate, attachment chemistry, formulation of the deposition buffer, and print climate needed to create arrays to meet your assay requirements.

MI scientific and engineering staff will help you define the parameters that best suit your array requirements. Please complete the Contact MI form so that we can contact you regarding the specific requirements for your protein arrays.